“The extraordinary property of the gut microbiome is that it’s malleable,” said the study’s senior author, Justin Sonnenburg, PhD, a professor of microbiology and immunology. “Because of this malleability, the gut microbiome can deteriorate. This is basically what has happened in industrialized nations over the past century.”
The researchers used 62 fecal samples collected over a year to study the infant gut microbes of the Hadza, a hunter-gatherer people in Tanzania, comparing them with those of 17 other populations around the world — including communities in Africa, Asia, North and South America, and Europe — using DNA sequencing, a method that profiles individual species from a sample.
Unearthing an underrepresented gut diversity
The Hadza of Tanzania, one of the last remaining full-time hunter-gatherer groups, have one of the least industrialized lifestyles in the world. The plentiful gut microbiomes of nonindustrialized people are crucial to understanding the true capacity of the gut, but they are understudied, Sonnenburg said.
Several factors have led to a low gut diversity in industrialized nations: cesarean sections; antibiotics; sanitation; And a diet higher in saturated fats, low in dietary fiber, and high in artificial sweeteners and emulsifiers, according to Sonnenburg. C-sections, which prevent the sharing of important vaginal bacteria, and baby formula go hand in hand with altering gut microbiome assembly early in life, he added.
There are greater than 100 times the amount of microbial genes in the human microbiome than human genes in the human genome: roughly 2 to 10 million genes. Because of the large number, the researchers used deep metagenomic sequencing to study the genomes of species within the microbial community — a method that provides insight into the functional capacity of all microorganisms.
The sequencing yielded a diversity not seen in industrialized nations: 23.4% of microbial species detected in the Hadza infants were novel species not found in the Unified Human Gastrointestinal Genome, a catalog of all bacteria that have been detected in the human gut microbiota.
“We have unlocked this reserve of gut diversity data that will forever change how we understand infant gut bacteria and their role in the human microbiome,” said Matthew Olmthe lead author of the study and a postdoctoral scholar in Sonnenburg’s lab.
The novelty provides foundational information for studying the functions that are central to infant gut microbiome development, including the functions that are being lost in industrialized populations.
Balancing microbes to support immune health
The team is particularly interested in the milk-digesting genes of the Bifidobacterium because their absence could have long-term immune consequences.
Milk sugars, called human milk oligosaccharides, feed and recruit beneficial bacteria, specifically B. infantis, which is the foundational species for building out the healthy gut microbiome of infants. Many common complications in the industrialized world, such as allergies and asthma, are thought to result from microbiome imbalances, according to Sonnenburg: The prevalence of one species over another may, in part, determine the trajectory of health for an infant.
Sonnenburg said that cataloging the variation in the gut microbiomes of humans around the world could help clinicians diagnose the cause of a disease and tweak a patient’s microbiome to ameliorate symptoms — what he calls precision health.
“If you change the gut microbiome, the changes ripple through the human body,” Sonnenburg said. “The same connections between the gut microbiome and all sorts of chronic inflammatory diseases — ranging from metabolic syndrome to heart disease to different types of cancer to autoimmune disease — also offer the potential to leverage these connections for precision health and ultimately disease prevention.”
The impact on the immune system of a lower amount of B. infantis, which infants often inherit from their mothers during childbirth and through breast milk, is largely unknown. Still, a University of Nebraska study suggest that a lack of B. infantis results in systemic inflammation and immune dysregulation early in life. Interventional studies, like one clinical study Sonnenburg pointed to that reintroduced B. infantis and found that malnourished children put on weight, are gaining interest among scientists like Sonnenburg.
Sonnenburg wants to see studies of microbiome diversity in more nonindustrialized populations, so he can learn more about interventional practices and understand the health implications of a changing microbiome.
“Gut microbiota are the drivers behind many functions within us,” Sonnenburg said. “Our gut microbiome is evolving, and we need to understand what it means to be losing these bacteria that may be important components of human biology.”
Researchers from the Chan-Zuckerberg Biohub, UC Berkeley and New York University Abu Dhabi contributed to this study.
This study was funded by the National Institutes of Health (grants DP1-AT009892, R01-DK085025, F32DK128865 and F32DK128865), the NSF Graduate Research Fellowship, the Stanford Graduate Smithship, and the Bill and Melinda Gates Foundation.